Journal: bioRxiv
Article Title: Life in the fast lane: Functional consequences of male-female dynamic differences in the renal autoregulation of flow
doi: 10.1101/2025.09.12.675896
Figure Lengend Snippet: (A) Intravital two-photon (2P) microscopy was used to quantify single-nephron tubular fluid flow dynamics in the kidney cortex. Representative time-lapse images with Lucifer Yellow (LY) or 70kDa dextran-rhodamine show real-time changes in tubular fluid flow dynamics in the kidney of an anaesthetized 3-month-old male C57BL/6J mouse. 2M dextran-rhodamine was used together with LY to label the circulating plasma. (B) A representative recording of 70kDa dextran-rhodamine fluorescence intensity within a luminal ROI of the early PT segment in an anaesthetized 3-month-old male C57BL/6J mouse. Raw signals were overlaid with a reference sine wave of fixed frequency as indicated. (C) Resting-state magnetic resonance imaging (MRI) was used to detect physiological oscillations at the multi-nephron spatial scale. With a resolution of 0.5×0.5×0.5mm 3 in a rat kidney, single-voxel recordings indicate collective fluid dynamics among about five adjacent nephrons in the renal cortex . The plot on the right represents a single-voxel time series obtained from a cortical region of an anaesthetized 3-month-old male Sprague-Dawley rat. Raw signals were overlaid with a reference sine wave. (D) Transit-time ultrasonic flow measurement was used to measure real-time renal blood flow through the renal artery. Data shown was collected from an anaesthetized 6-month-old male C57BL/6J mouse, previously reported in ref. . The original 1000Hz data was down-sampled to 2Hz and bandpass-filtered to visualize slow rhythms below 0.1 Hz, overlaid with a reference sine wave. (E) Glomerular filtration rate (GFR) can be estimated by fitting the transcutaneous clearance profile of a single intravenous bolus of FITC-sinistrin. Data shown was collected from an anaesthetized 3-month-old male C57BL/6J mouse, previously reported in ref. . The raw data points, with a 0.5 Hz sampling rate, are colored in red. Detrended signals for a 200-second interval are shown on the right, overlaid with a reference sine wave.
Article Snippet: The following antibodies were employed in this study recognizing KIM-1 (kidney injury molecule-1, Rat, 1:500, R&D Systems, MAB1817), FITC-conjugated LTL (Lotis Tetragonolobus Lectin, 1:1000, Vector Laboratories, FL-1321).
Techniques: Microscopy, Clinical Proteomics, Fluorescence, Magnetic Resonance Imaging, Filtration, Sampling
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